FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8818312
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG49850.081.007433
CCGAGTA53400.076.1083452
TACTCGG70400.076.019613
CTACTCG70750.074.723582
GAGTAGC121800.072.982654
GCTACTC163850.072.478121
AGTAGCT201100.071.85065
GCTGGGA473900.069.17179
GTAGCTG207950.068.920936
CCCGAGT64550.065.892221
TAGCTGG237800.062.8943947
AAGTGCT299300.062.268435
GGAGGCT395900.061.7919279
AAAGTGC296950.061.2721024
TCGGGAG90350.060.601366
TTGGGAG311950.060.5771756
CTCGGGA88000.059.9289445
CCAAAGT326000.059.8607182
CCCAAAG357350.059.5850751
ACTCGGG89300.058.692424
Produced by FastQC (version 0.11.5)