FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9361723
Sequences flagged as poor quality0
Sequence length100-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG65350.083.6861043
TACTCGG94100.079.131793
CCGAGTA70100.078.422662
CTACTCG93000.078.277942
AGTAGCT250050.077.3581855
GAGTAGC157450.077.136274
GCTACTC214100.075.613661
GTAGCTG260800.073.5939946
GCTGGGA612400.072.77419
TTGGGAG384900.067.617976
AAGTGCT380200.067.561295
AAAGTGC374300.067.057044
TAGCTGG300600.066.5203867
CCCGAGT84250.066.248941
GGAGGCT524650.065.9764259
TCGGGAG122400.065.9543156
CCAAAGT405400.065.6283342
CTCGGGA115450.065.459035
ACTCGGG113200.064.569374
CCCAAAG446750.064.193791
Produced by FastQC (version 0.11.5)