FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9361723
Sequences flagged as poor quality0
Sequence length100-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG92850.082.158023
CGAGTAG65200.081.068993
CTACTCG93600.080.453512
GCTACTC218000.078.6472551
AGTAGCT240750.078.49685
CCGAGTA68150.078.325382
GAGTAGC150450.078.0724264
GTAGCTG252000.075.018376
GCTGGGA611900.074.52299
AAGTGCT374700.070.863685
TTGGGAG400150.069.6997766
AAAGTGC373700.069.533734
CCAAAGT401800.068.860632
TAGCTGG289550.068.309287
GGAGGCT524750.068.1841059
CCCGAGT80600.067.775141
CCCAAAG444300.067.468351
CTACTTG140150.067.420272
CTCGGGA113400.067.039785
TCGGGAG119650.066.428476
Produced by FastQC (version 0.11.5)