FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10330685
Sequences flagged as poor quality0
Sequence length100-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG98450.081.621573
CGAGTAG63000.080.5912253
CTACTCG98700.079.930812
GCTACTC221000.078.4762951
GAGTAGC160050.077.457434
CCGAGTA67450.076.3619842
AGTAGCT260050.075.515975
GTAGCTG268750.072.468636
GCTGGGA655200.072.047999
GGAGGCT541800.067.809769
ACTCGGG118100.067.7431644
AAGTGCT425450.067.107615
TCGGGAG126800.066.899496
AAAGTGC416400.066.711044
CTCGGGA123200.066.127785
CCAAAGT449900.066.083532
TAGCTGG309150.064.7610247
TTGGGAG394350.064.651136
CCCAAAG498300.064.536251
CCCGAGT80200.064.376011
Produced by FastQC (version 0.11.5)