FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10330685
Sequences flagged as poor quality0
Sequence length100-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG100500.082.650933
CTACTCG99000.082.221532
GCTACTC223000.080.3742451
CGAGTAG62650.080.061053
GAGTAGC155800.078.62484
CCGAGTA66550.077.2713852
AGTAGCT253250.076.776415
GCTGGGA657600.075.169729
GTAGCTG266550.072.8560646
AAAGTGC420700.071.6934054
AAGTGCT429750.071.624775
CCAAAGT450150.070.244362
TCGGGAG129550.069.090576
GGAGGCT552500.068.966939
CCCAAAG495850.068.921041
ACTCGGG120200.067.9408954
CTCGGGA125850.067.166335
CAAAGTG459050.067.082823
AGTGCTG450300.067.026246
TAGCTGG300100.066.946897
Produced by FastQC (version 0.11.5)