FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14933478
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG72400.079.7220153
CTACTCG121000.079.312892
TACTCGG125000.078.480993
GCTACTC280750.077.0802461
CCGAGTA78650.074.6988752
GAGTAGC184900.073.891974
GCTGGGA787050.073.0471049
AGTAGCT305350.070.405575
AAGTGCT553600.067.738425
AAAGTGC542800.067.577994
GTAGCTG320100.067.440726
TCGGGAG159900.067.202816
GGAGGCT678350.066.85269
CCCAAAG644650.065.526331
CCAAAGT591450.065.264522
CTCGGGA157850.064.676165
AGTGCTG573800.064.334666
GTGCTGG580950.062.9389427
ACTCGGG156000.062.576714
TTGGGAG488600.062.2334256
Produced by FastQC (version 0.11.5)