FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3564170
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11800.031.075963
CCGAGTA13600.028.694182
AGTAGCT48450.027.6543245
CGCGAGT2053.45608E-1127.5937771
GCTGGGA84600.027.440879
GAGTAGC30250.026.4201624
GTAGCTG48800.026.2036066
TAGCGCG909.5070264E-426.1179715
GGAGGCT64650.025.877539
TACTCGG10750.025.3648053
GCTACTC27450.024.9005781
TTGGGAG65400.024.8000936
CCCGAGT18450.024.5278021
GGGAGGC82700.023.9231248
CTACTCG10850.023.8334872
TAGCTGG54550.023.6987557
TCGGGAG14250.023.0937866
CTCGGGA12700.021.8403845
CGGAGGT12400.021.6093037
CCGTACG550.003060160221.36475272-73
Produced by FastQC (version 0.11.5)