FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3564170
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG13200.036.6557353
CTACTCG12450.036.5999952
CGAGTAG12550.032.9395453
CCGAGTA13050.032.3974382
GAGTAGC31850.031.2683584
GCTACTC29050.031.2636661
AGTAGCT48400.030.5734085
GTAGCTG50050.029.659356
GCTGGGA90550.029.4152989
GGAGGCT71150.029.182749
TTGGGAG70550.029.0314086
CGGAGGT14100.028.6522037
TCGCGTT1005.34498E-528.1857685
ACGGTCG1005.34498E-528.1857683
TAGCTGG55200.027.9134457
CTCGGGA17050.027.5520735
CCCGAGT18000.027.45021
ACTCGGG19300.027.0174484
TCGGGAG17300.026.8823816
CGCGTTG1101.022426E-425.6234266
Produced by FastQC (version 0.11.5)