FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8089521
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG43250.074.848543
CCGAGTA45650.073.461252
GAGTAGC107150.069.469594
CTACTCG56350.069.3734362
TACTCGG58000.069.1823653
AGTAGCT179300.068.795515
GCTACTC135400.066.294031
GCTGGGA409850.065.473589
GTAGCTG183200.064.9678656
CCCGAGT58100.062.510221
GGAGGCT333600.061.399149
TAGCTGG203700.060.1414227
TTGGGAG277550.059.039476
AAAGTGC256500.058.896784
AAGTGCT260450.058.871875
CCAAAGT281550.058.1085322
TCGGGAG74500.057.589896
CCCAAAG311150.057.2608451
AGTGCTG268200.056.327466
CTCGGGA71650.056.002815
Produced by FastQC (version 0.11.5)