FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3063156
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG8800.034.1946752
CCGAGTA10750.033.677782
AGTAGCT36950.032.1881755
TACTCGG9800.032.1394923
CGAGTAG10700.032.0722433
TTGGGAG55750.029.934626
GAGTAGC26200.029.426114
GTAGCTG38700.029.274966
GGAGGCT55700.028.4330339
TCGGGAG12300.028.2824426
GCTGGGA69800.027.6716379
GGGAGGC72600.027.6401068
CCCGAGT15900.027.5580641
CTCGGGA12000.027.42255
GCTACTC24950.027.0040151
CGTAACG350.00884245126.85496526-27
CGGAGGT9750.026.5171667
GGAGGTT28450.025.933788
TAGCTGG44900.025.5453857
CTCGTAT16050.024.89016794
Produced by FastQC (version 0.11.5)