FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3063156
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG9400.035.9847833
AGTAGCT39950.034.4560175
CTACTCG9450.034.3029562
GTAGCTG39600.034.167376
CGGAGGT11850.033.3023767
GCTGGGA74200.032.3542449
GAGTAGC26200.031.9178034
CGAGTAG9650.031.644653
TTGGGAG58350.031.6421456
CCGAGTA10100.031.630192
GCTACTC23400.031.5646571
TAGCTGG46800.030.517017
CTCGGGA11550.029.693075
TCGGGAG13350.029.5605346
GGAGGCT60100.029.4700669
CCCGAGT14200.029.1549021
TCGCGTT650.00616847128.9108545
GGAGGTT34450.028.910858
AGGCGGA18100.027.2536684
GGCGGAG18150.027.1785895
Produced by FastQC (version 0.11.5)