FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8171431
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG31850.059.7104383
CTACTCG33050.057.93172
GCTACTC84550.051.8546261
GCTGGGA245750.050.4454659
CCGAGTA27750.049.843362
CGAGTAG27100.049.61963
GGAGGCT198150.048.262419
GAGTAGC69950.046.218854
CCCGAGT37850.045.5545161
TCGGGAG43000.045.5243876
CTCGGGA41050.045.226715
AGTAGCT118250.043.3652155
GTAGCTG118500.043.187976
CGGAGGT42000.042.645257
GTGCTGG169600.042.054357
TTGGGAG181950.041.980096
CCCAAAG220950.041.71671
AGTGCTG179800.040.70316
AAAGTGC185200.040.410014
TAGCTGG134850.040.273437
Produced by FastQC (version 0.11.5)