FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8171431
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG34850.057.6732753
CTACTCG35900.055.629172
GCTACTC88900.053.294731
CGAGTAG27550.052.50913
CCGAGTA29750.051.422522
GCTGGGA249800.050.8212369
GGAGGCT205650.050.8104939
GAGTAGC70300.047.6199344
CCCGAGT38300.046.974471
TTGGGAG187100.046.0717856
CGGAGGT47000.045.744627
AGTAGCT117400.045.5798235
GTAGCTG118400.045.1851586
CTCGGGA45050.045.0400545
TCGGGAG47050.044.476376
GTGCTGG176950.043.6470957
TCGCGTT2800.043.4340175
CCCAAAG221550.042.886091
AGTGCTG180550.042.8518946
AAAGTGC182450.042.6260264
Produced by FastQC (version 0.11.5)