FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10066470
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG57150.084.569843
TACTCGG90500.081.478043
CCGAGTA60050.080.7089542
GAGTAGC145700.079.686114
AGTAGCT235200.079.507745
CTACTCG91400.079.282252
GCTACTC211550.079.041941
GTAGCTG246050.076.0164956
GCTGGGA591450.075.520399
GGAGGCT499550.071.906469
TCGGGAG113750.069.577656
TAGCTGG280350.069.0843057
CCCGAGT71950.068.86331
CTCGGGA108400.068.819015
GCTACTT159300.066.625311
TTGGGAG369650.066.461956
AAGTGCT398350.066.3107765
ACTCGGG110500.065.941914
AAAGTGC394300.065.826824
CCAAAGT417550.065.773952
Produced by FastQC (version 0.11.5)