FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15173704
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG68850.074.6218953
TACTCGG105450.074.218083
CTACTCG106050.073.024072
CCGAGTA75400.069.5004652
GAGTAGC173900.068.962444
GCTACTC255400.068.827761
GCTGGGA719700.067.612999
AGTAGCT290400.065.7025455
TCGGGAG131850.064.494086
GTAGCTG304500.063.3564226
GGAGGCT603450.062.136589
CCCGAGT89750.061.373641
CTCGGGA132100.060.8506665
AAAGTGC497450.060.758434
AAGTGCT507500.060.040525
CCCAAAG598350.058.8369981
CCAAAGT547800.058.5458152
ACTCGGG134200.058.5220684
AGTGCTG520650.057.9457446
TAGCTGG343800.057.9049877
Produced by FastQC (version 0.11.5)