FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2985439
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGAG65050.036.687346
GTAGCTG39450.035.8442346
AGTAGCT39400.035.772885
CGAGTAG9450.035.2978443
CCGAGTA9900.034.6425062
GGAGGCT61800.034.5877849
GAGTAGC24550.034.4463044
GCTGGGA75850.033.6313329
CGGAGGT12350.033.0942157
TAGCTGG44700.031.8445347
GGGAGGC84400.031.7828948
GCACTTT56050.031.6365831
TACTCGG8900.031.1446633
CTACTCG9300.030.8154492
GCTACTC23100.030.5423981
TTTGGGA66500.030.3085045
GGAGGTT32200.030.0546138
TGGGAGG85350.029.5576957
ACTTTGG61100.029.1420173
CACTTTG61350.029.0998422
Produced by FastQC (version 0.11.5)