FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3082498
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG7100.037.1107182
TACTCGG7450.035.997073
CGAGTAG9650.034.1286623
CCGAGTA10150.032.9125822
TTGGGAG58700.032.907976
GAGTAGC24950.031.865624
TCGCGTT902.5787072E-531.362815
AGTAGCT39500.031.204015
GTAGCTG39550.031.1352396
GCTACTC20300.030.8546351
GCTGGGA72200.030.332699
GGAGGCT56000.029.7921949
ATGCGCG804.7564696E-429.4026344
GGGAGGC70300.029.081538
TAGCTGG45050.027.8547847
CGGAGGT8900.026.9316837
TGGGAGG74050.026.656777
CCCGAGT15100.026.1979431
GCACTTT52500.025.834381
TTTGGGA64400.025.5675075
Produced by FastQC (version 0.11.5)