FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3082498
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG8500.037.617142
CGGAGGT14000.036.5857857
TACTCGG8700.036.2119063
CCGAGTA10300.033.7823832
GCTACTC22100.033.4298671
GAGTAGC25950.033.159084
TTGGGAG64350.033.0777056
GGAGGCT61250.032.684259
GCTGGGA71800.032.397829
AGTAGCT39300.032.300085
CGAGTAG10100.032.123553
CCCGAGT13400.030.9033221
GTAGCTG40750.030.787146
GGGAGGC81750.029.9487028
GGAGGTT32650.029.7930648
CGTACGA404.7873295E-429.3707572-73
GCGGAGG17000.029.2983176
TAGCTGG44550.028.0574287
CTCGGGA12450.027.9443975
TGGGAGG81900.027.0814537
Produced by FastQC (version 0.11.5)