FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3160338
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN89090.2819002271276047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG9250.029.9872593
GCTGGGA67000.029.2932919
CGAGTAG10050.029.0036143
CCGAGTA10800.028.7293592
CTACTCG8550.028.5918982
CTCGCGT1354.628946E-727.8587724
TTGGGAG50800.027.8362856
GAGTAGC23800.027.653934
CCGGGTT7650.027.0058568
GTAGCTG38600.026.8975966
AGTAGCT37800.026.8629635
CGGAGGT10900.026.7099677
AGGCGGA15450.026.4725974
CCCGAGT15000.026.3483561
GGCGGAG14950.025.47045
TAGCTGG43450.024.9649287
CGGGTTC7150.024.9542359
CTCGGGA11150.024.8753455
TCGGGAG12300.024.8265726
GGAGGCT51050.024.6493649
Produced by FastQC (version 0.11.5)