FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8513993
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA38250.068.251082
CGAGTAG37600.068.189313
TACTCGG50250.067.902143
CTACTCG51550.064.967752
GCTACTC122300.061.7158241
GAGTAGC97350.060.3891374
GCTGGGA352350.058.59929
AGTAGCT159800.057.533465
CCCGAGT52200.057.0180241
GGAGGCT292350.055.898199
GTAGCTG163200.055.5810666
TCGGGAG65600.053.9873966
CTCGGGA63450.053.5718465
TTGGGAG240350.052.394346
AAAGTGC233450.051.502344
CCCAAAG285500.051.4209751
ACTCGGG65500.051.005544
TAGCTGG183800.050.6902247
AAGTGCT240000.050.6362955
CCAAAGT262750.049.826412
Produced by FastQC (version 0.11.5)