FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8513993
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG51300.075.591533
CGAGTAG35500.073.3096853
CTACTCG51950.073.0256962
CCGAGTA39300.068.527432
GCTACTC126600.068.176411
GAGTAGC95350.062.464144
GCTGGGA353350.062.4217349
CCCGAGT51100.061.4551771
AGTAGCT155750.061.018525
TCGGGAG67950.060.118576
CTCGGGA64000.060.0855645
GGAGGCT300100.059.842439
GTAGCTG162100.058.4691546
ACTCGGG67150.057.652594
AAAGTGC226000.056.689294
AAGTGCT233350.055.8747185
TTGGGAG249900.055.1541526
CCCAAAG281750.054.511531
TAGCTGG181950.054.189687
AGTGCTG237700.053.572756
Produced by FastQC (version 0.11.5)