FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10409960
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG78200.075.184443
CTACTCG78850.073.585342
CGAGTAG48300.073.5320363
GCTACTC189000.069.556751
CCGAGTA52700.069.3167652
GCTGGGA497850.065.0019459
GAGTAGC128400.064.227714
GGAGGCT421550.062.792339
AGTAGCT214650.062.649275
TCGGGAG97850.062.38096
CTCGGGA96400.061.4704555
GTAGCTG219150.060.894166
ACTCGGG96500.059.0635154
CCCGAGT67500.058.5362471
AAGTGCT343900.057.668845
AAAGTGC338050.057.584764
TTGGGAG329200.057.463526
CCCAAAG407550.056.6658821
GTGCTGG344550.055.906717
AGTGCTG347800.055.766926
Produced by FastQC (version 0.11.5)