FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10409960
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG77500.078.006542
TACTCGG78550.077.471823
CGAGTAG51200.075.4701163
GCTACTC185750.074.9844061
CCGAGTA54700.073.437592
GAGTAGC123700.069.087614
GCTGGGA500800.068.6132669
AGTAGCT207300.066.17755
GGAGGCT439450.065.64939
TCGGGAG98550.065.576636
CTCGGGA95150.064.576365
CCCGAGT68050.064.316271
GTAGCTG212200.063.6675036
TTGGGAG341300.062.844546
ACTCGGG97000.062.2559554
AAAGTGC338250.060.7754144
AAGTGCT342450.060.7213135
CCCAAAG403600.059.8738751
CCAAAGT369800.059.7270162
GTGCTGG350800.058.3947337
Produced by FastQC (version 0.11.5)