FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7648332
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG40100.072.124243
CCGAGTA42350.070.1629642
TACTCGG49050.067.237293
CTACTCG49550.066.341762
GAGTAGC97900.065.532384
AGTAGCT155500.065.159485
GCTGGGA354750.063.1886339
GCTACTC116700.063.1375471
GTAGCTG160850.062.9922186
CCCGAGT51250.062.44251
GGAGGCT279000.058.1575439
TAGCTGG181550.057.725887
TCGGGAG63350.056.4320226
AAAGTGC225000.056.0543674
TTGGGAG236450.054.5386056
AAGTGCT235850.054.294995
CCCAAAG280000.053.6204951
CTCGGGA61850.053.114095
GTGCTGG233700.053.0029227
CCAAAGT258350.052.6422422
Produced by FastQC (version 0.11.5)