FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7648332
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG39100.074.7841643
TACTCGG52350.072.588173
CCGAGTA41150.071.2132342
CTACTCG52950.069.574362
GAGTAGC95800.068.0370564
AGTAGCT151150.067.794655
GCTACTC127400.066.589621
GCTGGGA358050.066.395629
GTAGCTG159750.064.144986
GGAGGCT297450.062.578189
TCGGGAG67000.059.888946
CCCGAGT51800.059.814361
AAAGTGC227900.059.5740054
TTGGGAG241500.059.473176
CTCGGGA63200.059.209645
AAGTGCT233900.058.596925
TAGCTGG182550.058.2856457
CCCAAAG278450.057.2093541
AGTGCTG237200.056.8856476
CCAAAGT257050.056.825682
Produced by FastQC (version 0.11.5)