FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3664661
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT13350.035.901857
TCGCGTT1204.2000465E-935.261145
TTGGGAG67850.034.2781756
GGAGGCT67500.033.8368349
CTACTCG10400.033.45472
CCGAGTA11000.033.3396222
GCTACTC25500.032.848261
CGAGTAG10450.032.3947753
TACTCGG10750.031.4907363
GGGAGGC90950.030.690188
GCTGGGA79400.030.186099
GAGTAGC28500.029.8601844
AGTAGCT43300.029.7507365
GTAGCTG44000.028.9386986
GCGGAGG17150.028.4925676
CTCGGGA13300.028.2796065
TGGGAGG90600.028.1105277
GCACTTT61800.027.7930931
TAGCTGG47800.027.6233487
TTTGGGA72400.027.5984975
Produced by FastQC (version 0.11.5)