FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8655609
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG40500.069.358523
TACTCGG47650.066.035933
CCGAGTA43900.065.4751742
CTACTCG48000.063.6002662
GAGTAGC103400.060.86294
AGTAGCT168800.060.4891245
GCTACTC117700.059.5686651
GTAGCTG173400.058.4436456
GCTGGGA365400.058.310449
CCCGAGT57950.056.805831
TAGCTGG189750.054.951957
TCGGGAG63150.052.9544146
GGAGGCT292300.052.561439
CTCGGGA61100.051.8121265
ACTCGGG62350.050.569074
TTGGGAG245550.049.9871066
AAAGTGC234400.048.9952934
AAGTGCT239550.048.7396625
CCCAAAG283750.048.0019261
CGGAGGT62050.047.733957
Produced by FastQC (version 0.11.5)