FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938479
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAA450.001014544541.7750194
CCGTACG704.5462566E-640.4026761
CCGAGTA11250.036.3603442
CGAGTAG10700.036.031023
TTGGGAG58750.033.691536
CGTACGT702.1810018E-433.5840642
TACTCGG8550.032.9937443
AGTAGCT40100.032.829195
CTACTCG8350.032.0959052
GAGTAGC25300.031.9635054
GCTGGGA72300.031.7841119
GGAGGCT57300.030.8370469
GTAGCTG41600.030.7412856
GCTACTC20900.030.6725271
CCCGAGT14050.030.194171
GGGAGGC74100.030.060748
TAGCTGG45450.029.1657417
ACGATCG650.00615974828.9191918
TTTGGGA63500.027.247125
GCACTTT54250.027.0220071
Produced by FastQC (version 0.11.5)