FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938479
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG9350.036.6778563
CGAGTAG10800.035.668333
CCGAGTA11100.035.5513762
TTGGGAG64000.035.4517486
CTACTCG9550.035.4184232
CGGAGGT11650.034.27397
AGTAGCT39750.034.0367475
GTAGCTG40850.033.463516
TAGCTGG44550.032.8985987
GGAGGCT60850.032.8872039
GCTGGGA72750.032.350629
GAGTAGC25300.031.9374774
GGGAGGC83350.031.8429558
GCACTTT54900.031.6296041
TCGGGAG13500.030.6210046
TTTGGGA66950.030.5232935
CTCGGGA13000.029.2707825
CCCGAGT14700.029.1315631
ACTTTGG60400.029.0111263
GGAGGTT34050.028.8336548
Produced by FastQC (version 0.11.5)