FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11187878
Sequences flagged as poor quality0
Sequence length100-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG73050.084.563323
CCGAGTA77350.080.6473162
GAGTAGC178200.079.6890954
AGTAGCT284350.078.396285
TACTCGG100600.076.706233
GCTACTC233500.076.2044141
CTACTCG98300.075.700082
GTAGCTG301000.074.298766
GCTGGGA722700.074.109649
TAGCTGG339650.067.7589657
CCCGAGT91750.067.701951
AAGTGCT471800.066.692455
AAAGTGC462600.066.305124
GGAGGCT562400.066.0288549
CCAAAGT504450.065.721462
CCCAAAG552400.065.10561
TCGGGAG128900.063.6351476
CAAAGTG503850.063.1025053
TTGGGAG414700.062.6108746
AGTGCTG498350.062.319476
Produced by FastQC (version 0.11.5)