FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10566546
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG55550.070.2794342
TACTCGG57750.069.309243
GCTACTC139800.063.9492871
CCGAGTA44600.063.808552
CGAGTAG42600.063.7186133
GCTGGGA394100.060.6875429
CCCGAGT57000.057.43261
GGAGGCT331000.056.8723569
GAGTAGC104650.056.8375324
TCGGGAG71300.056.5807956
CTCGGGA68450.056.0662575
AGTAGCT171450.055.040095
GTAGCTG174100.053.4762956
GTGCTGG279850.053.178647
ACTCGGG73900.053.095264
AAGTGCT289550.052.880375
TTGGGAG277600.052.851766
AAAGTGC285500.052.6945344
CCCAAAG347400.052.376961
AGTGCTG288200.052.2529566
Produced by FastQC (version 0.11.5)