FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2931584
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT602.5682311E-854.805345
CTACTCG8650.038.015262
CCGAGTA10550.037.402692
TACTCGG8950.036.741013
CGGAGGT14550.036.482467
CGAGTAG9950.036.3532873
CGCGTTG959.4964344E-734.6133046
GCTACTC22200.034.548551
TTGGGAG64250.034.3631826
GCTGGGA75150.033.567119
GGAGGCT62850.032.0642179
GTAGCTG40800.031.3168036
AGTAGCT41100.031.2030375
GAGTAGC25550.030.888334
GGAGGTT38350.029.7652518
GGGAGGC85750.029.6368338
CCCGAGT14600.029.0057981
ATTCGCG650.006171080728.908314
TAGCTGG45500.028.8045737
CCCAAAG53900.028.4592881
Produced by FastQC (version 0.11.5)