FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3324434
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG11000.040.1514742
TACTCGG11350.038.0859573
CGAGTAG12200.037.7432373
AGTAGCT45700.036.0886275
CCGAGTA13100.035.8669972
TTGGGAG69950.035.802966
GAGTAGC30300.035.5117654
GCTACTC25200.035.246221
GTAGCTG48550.034.7443856
GCTGGGA89950.034.267999
GGAGGCT68350.032.6543469
TAGCTGG53450.031.9987557
CCCGAGT17050.031.973111
GGGAGGC94250.031.3584988
CGCCGTA902.5930221E-531.33751994
CTCGGGA14400.031.324795
GCACTTT61050.030.714151
CACTTTG63450.029.9909152
ACTTTGG63600.029.6993
AAAGTGC48150.029.6658424
Produced by FastQC (version 0.11.5)