FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7217345
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG43500.079.310473
CCGAGTA46000.076.820752
GAGTAGC107850.073.9322364
TACTCGG54500.073.105353
CTACTCG55850.071.109242
AGTAGCT177050.070.915485
GCTACTC126750.070.442011
GCTGGGA408800.068.97949
GTAGCTG184550.068.0560766
CCCGAGT54900.067.118191
TAGCTGG203550.063.912457
AAAGTGC247100.061.6204264
AAGTGCT256150.061.1010865
GGAGGCT312150.060.9596259
CCCAAAG301700.059.252161
CCAAAGT274950.059.0178152
TCGGGAG72250.058.568866
TTGGGAG241400.058.209376
CTCGGGA71400.057.6848145
ACTCGGG69750.057.4812974
Produced by FastQC (version 0.11.5)