FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11213059
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG93900.079.1107252
TACTCGG96350.078.065423
CGAGTAG57000.075.371213
GCTACTC217950.073.829431
CCGAGTA62100.070.787682
GAGTAGC145100.069.8495254
GCTGGGA584650.068.738019
AGTAGCT232600.068.384035
CTCGGGA113100.065.850445
GTAGCTG241050.065.424616
GGAGGCT523550.064.834479
AAGTGCT389200.064.2589655
TCGGGAG121400.064.022026
AAAGTGC384400.063.2684864
ACTCGGG117850.062.751064
TTGGGAG389100.062.3684846
CCCAAAG461650.061.4981541
CCAAAGT422600.061.310842
CCCGAGT76400.061.034471
AGTGCTG403650.060.795676
Produced by FastQC (version 0.11.5)