FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855422
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT901.2842065E-841.889975
CTCGCGT1204.101821E-935.3452844
TACTCGG7850.032.4186023
AGTAGCT40400.032.0784875
CTACTCG7800.031.4229972
GCTGGGA69300.031.3267049
CCGAGTA11200.031.1424312
GAGTAGC25200.031.0440034
GTAGCTG40250.030.714286
CGAGTAG11050.030.7072123
TTGGGAG57200.029.8305386
CGGAGGT9150.029.7860457
GGAGGCT50500.028.473189
CCCGAGT13600.028.4238191
TAGCTGG45600.028.1322027
GGGAGGC68400.027.6856618
CGCGTTG1555.082984E-827.2936296
CCCAAAG54750.026.0034081
TGGGAGG73350.025.4969887
AAGTGCT41200.024.7069475
Produced by FastQC (version 0.11.5)