FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855422
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN81800.2864725424122949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGC25350.036.9188044
GCTGGGA69400.036.0703859
AGTAGCT42550.036.0296365
CCGAGTA11300.035.7925382
GTAGCTG43600.035.3464976
CGAGTAG11100.035.1676183
TACTCGG8700.034.0572133
TAGCTGG46650.033.9306267
CTACTCG8250.033.6333852
CCCGAGT14150.033.598561
TTGGGAG62500.033.0022476
CGGAGGT11300.032.0055927
GCTACTC20600.031.761671
GGAGGCT55050.030.6281079
CGGGTTC6900.028.5879639
GGGAGGC82300.027.6207988
GCACTTT54300.027.566591
GGAGGCC43850.027.3120069
TGGGAGG78450.027.1218227
CCCAAAG52300.026.9107151
Produced by FastQC (version 0.11.5)