FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3896940
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG18150.042.9847953
AGTAGCT69750.042.652345
CCGAGTA18900.041.030382
GTAGCTG72800.040.4780466
GAGTAGC45300.039.6322364
GCTGGGA130550.039.3483629
TAGCTGG80700.037.8549967
TTGGGAG97150.037.153686
TACTCGG13850.036.9879343
CTACTCG13300.036.7506562
CCCGAGT22600.036.2497521
AAAGTGC63450.035.554374
CCCAAAG78900.035.0884781
GCACTTT76950.034.937491
AAGTGCT66600.034.6489945
GCTACTC31400.034.4875531
CCAAAGT72250.034.0860632
GGAGGCT94800.034.0094159
AAGTAGC32650.032.531844
GGGAGGC129100.032.5093738
Produced by FastQC (version 0.11.5)