FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3896940
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA17950.048.1637342
CGAGTAG17600.047.5197453
AGTAGCT66300.045.2849735
GTAGCTG66550.044.3382346
GAGTAGC42850.043.9703944
TTGGGAG103650.042.2487076
CCCGAGT22950.040.748841
CTACTCG14750.040.4555852
GCTGGGA131450.040.3899659
GCACTTT84850.039.9326631
TAGCTGG78450.039.767737
TACTCGG14850.039.233953
GCTACTC36500.038.8829231
AAAGTGC63800.037.338264
TTTGGGA100550.036.9624945
ACTTTGG91850.036.1665463
GGAGGCT103150.036.029899
CACTTTG94000.035.8391842
AAGTGCT67300.035.6058965
CCCAAAG80500.035.3770451
Produced by FastQC (version 0.11.5)