FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4095075
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG11950.031.1372
TACTCGG13000.030.4283983
CCGAGTA15200.029.747142
CGAGTAG14800.029.5913263
AGTAGCT53000.029.2319585
CCCGAGT19550.028.4332961
GCTACTC30700.028.2339361
GTAGCTG54400.027.7331986
GAGTAGC35050.027.0054384
GCTGGGA97650.026.5645399
GGAGGCT72650.025.8737779
TTGGGAG78500.025.5054936
TAGCTGG64000.024.6714557
GGGAGGC95750.024.0487658
CGGAGGT12500.023.6845977
CTCGGGA16700.022.2765775
TGGGAGG101900.022.0439367
GCACTTT65350.021.9861
TCGGGAG17300.021.73396
CGTACGG550.003063244521.3610938-39
Produced by FastQC (version 0.11.5)