FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4095075
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN117190.2861730249140736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG12750.036.130582
TACTCGG13250.034.7693023
GCTGGGA93250.031.522099
CCGAGTA16300.031.4338782
CGAGTAG15950.030.9467033
TCGGGAG16850.030.3895426
CGGAGGT15400.030.1892287
GGAGGCT79450.029.4323529
CTCGGGA17150.029.3281085
GCTACTC32500.029.2383521
GTAGCTG54400.029.1023856
TTGGGAG76050.028.847976
AGTAGCT55550.028.1789385
CCCGAGT22300.028.0563811
GAGTAGC36550.028.0378174
GGGAGGC102700.026.6101558
TAGCTGG63400.026.0729947
CCCGAAT8050.025.1279721
AGGCGGA23800.025.0842934
GGAGGTT43600.024.6628848
Produced by FastQC (version 0.11.5)