FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3302004
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGT952.0814696E-839.65924
CCGTACG250.001703738637.5949472-73
TCGCGTT906.0981984E-736.6291165
CGCGTGA702.1600367E-433.6394963
CTACTCG8500.029.3697592
TACTCGG8750.028.5262933
AGTAGCT39650.027.080875
TAGCGGG2104.7293724E-1126.9111885
TTGGGAG53550.026.505916
GGAGGCT55200.026.3907749
GCTGGGA65850.025.8334459
GTAGCTG40800.025.458186
GAGTAGC24750.025.117494
GGGAGGC69200.024.5828348
CGGAGGT8850.024.4255337
GCTACTC22000.023.9848841
TAGCTGG44000.023.6031047
GGAGGTT24850.023.259928
GGCGGAG13200.022.8337355
TGGGAGG70850.022.816457
Produced by FastQC (version 0.11.5)