FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2846637
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT48750.045.163765
CGAGTAG13400.043.8881233
GTAGCTG49750.043.647126
GAGTAGC31950.043.1435244
CCGAGTA14450.041.0289422
TAGCTGG54950.040.371367
TACTCGG9700.040.257623
GCTGGGA92900.039.610199
CCCGAGT16800.039.5375251
CTACTCG9700.038.806622
TTGGGAG69800.037.169816
GCTACTC23350.037.121931
GCACTTT59000.035.610861
CCCAAAG55850.035.5949941
GGAGGCT65900.035.157869
CGGAGGT12450.034.731027
AAAGTGC44800.033.3939634
CAAGTAG21700.033.172143
TTTGGGA70050.032.8412445
GGGAGGC89950.032.500378
Produced by FastQC (version 0.11.5)