FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2846637
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG10150.049.092263
CTACTCG10400.047.912162
AGTAGCT48100.047.7883155
GAGTAGC33050.046.5103344
GTAGCTG50200.045.673096
GCTACTC25500.045.5678251
CCGAGTA12650.044.964322
CGAGTAG12700.044.7872963
TTGGGAG75450.044.3368726
GCTGGGA90550.043.126169
TAGCTGG56100.042.0443847
GGAGGCT74500.040.6216749
GCACTTT66250.039.481211
CCCGAGT16150.039.324461
TTTGGGA73500.038.9482465
TCGGGAG15000.038.5263756
CTCGGGA13300.037.817265
AAAGTGC45250.037.294874
CCCAAAG60350.037.26081
GGGAGGC108450.036.7421048
Produced by FastQC (version 0.11.5)