FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9740176
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG38250.064.42093
CTACTCG40700.057.9955862
CCGAGTA33050.054.938222
CGAGTAG31900.053.461323
GCTACTC100250.052.8350871
CTCGGGA49100.051.0990265
GAGTAGC83450.050.342154
GCTGGGA294050.050.146159
GGAGGCT239650.049.634299
TCGGGAG52550.049.071366
CCCGAGT46100.048.7182661
CGGAGGT52000.048.4655047
AGTAGCT143600.045.0566865
ACTCGGG53000.044.8924524
GTAGCTG147000.044.4284256
TTGGGAG207750.043.256686
TAGCTGG157800.042.9448627
CCCAAAG266900.040.9553071
GGGAGGC281000.040.4398738
GTGCTGG208300.040.2468157
Produced by FastQC (version 0.11.5)