FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3304430
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA12100.039.6599732
CGAGTAG11800.037.076533
AGTAGCT44000.036.7765
CCCGAGT15450.034.756341
GCTGGGA83700.034.699829
TTGGGAG67850.034.634646
GAGTAGC30750.034.4188274
GTAGCTG46600.033.585276
CTACTCG8300.031.176132
TAGCTGG51900.030.969177
GGGAGGC83800.030.9574998
TACTCGG9150.030.8480913
GGAGGCT63950.030.7184989
GCTACTC22900.030.442751
TCGCGTT1102.951494E-629.933955
TTTGGGA69800.028.9782225
CCCAAAG60650.028.1924921
AAAGTGC48500.028.1264534
CGGAGGT11900.028.0402687
GTGCTGG50000.028.0102547
Produced by FastQC (version 0.11.5)