FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3304430
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG10250.044.028283
CGAGTAG12150.041.3991853
CTACTCG9900.041.3112532
CCGAGTA12550.040.4542662
TTGGGAG69250.037.9935046
AGTAGCT46300.037.3638045
GCTGGGA84550.036.738469
GTAGCTG45850.036.684686
GCTACTC26650.036.543161
GGAGGCT68900.035.6710249
CGCCGTA1750.034.9154294
GAGTAGC29650.034.8804664
CGGAGGT14600.034.437467
GCACTTT57550.034.0079881
CCCGAGT16150.033.5010381
TAGCTGG51250.033.3739627
GGGAGGC95300.031.8029238
CTCGGGA13850.031.565855
TTTGGGA70050.031.4737875
TCGGGAG14950.030.7982466
Produced by FastQC (version 0.11.5)