FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14188913
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG62300.064.501122
TACTCGG63250.064.4717563
CGAGTAG44450.058.269293
GCTACTC166650.056.1773721
CCGAGTA49800.055.1525462
TCGGGAG79950.052.6965686
CTCGGGA79500.051.378635
GCTGGGA464100.051.28869
GGAGGCT380750.050.2438359
CCCGAGT67850.048.422881
GAGTAGC123050.047.9783554
ACTCGGG86350.046.5980074
AGTAGCT215500.044.152935
CGGAGGT89350.043.2155957
GTAGCTG219650.043.1647766
CCCAAAG429450.042.6533161
GTGCTGG340950.042.5833977
AAAGTGC347650.041.9391144
TTGGGAG326250.041.8502926
TCGCGTT4850.041.830425
Produced by FastQC (version 0.11.5)