FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11928284
Sequences flagged as poor quality0
Sequence length100-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG93150.086.406293
CCGAGTA98100.083.982952
AGTAGCT357800.080.2577745
GAGTAGC221450.080.157554
GTAGCTG377400.076.685466
TACTCGG128150.075.915723
CTACTCG127200.075.278722
GCTGGGA901200.071.750039
GCTACTC287050.070.813851
CCCGAGT122900.067.850451
TAGCTGG447000.067.3639767
AAGTGCT523850.066.306395
AAAGTGC521050.065.558434
CAAGTAG143250.065.374183
TTGGGAG536950.064.7749256
GGAGGCT706350.064.015229
CCAAAGT574150.063.7137832
TCGGGAG168700.063.430936
CTCGGGA162300.062.346285
CAAAGTG569450.062.332373
Produced by FastQC (version 0.11.5)