FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11928284
Sequences flagged as poor quality0
Sequence length100-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG93500.086.004913
CCGAGTA98200.083.57182
AGTAGCT347500.083.429355
GAGTAGC218350.082.6520164
GTAGCTG362700.080.1517946
TACTCGG136500.074.899363
CTACTCG132550.074.8360752
GCTGGGA876300.074.130699
GCTACTC292150.072.4653241
TAGCTGG424400.071.8805167
CCCGAGT119450.069.875291
TTGGGAG547900.068.653296
AAGTGCT501250.068.076695
AAAGTGC501550.067.165744
CCAAAGT537150.066.456392
GGAGGCT707800.066.0471659
GCACTTT363350.065.764761
ACTTTGG367700.065.1952063
CCCAAAG597150.064.89191
CTACTTG176300.063.8420332
Produced by FastQC (version 0.11.5)